phusion high fidelity master mix (New England Biolabs)

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New England Biolabs phusion high fidelity master mix
Phusion High Fidelity Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/Phusion+High-Fidelity+PCR+Master+Mix+with+HF+Buffer/pmc13691489-72-22-26
Average 99 stars, based on 6470 article reviews

phusion high fidelity master mix - by Bioz Stars, 2026-11

99/100 stars

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Article Title: Rhipicephalus microplus and Its Impact on Anaplasma marginale Multistrain Infections in Contrasting Epidemiological Contexts
Article Snippet: .. Molecular amplifications were performed on a T21 thermal cycler (IVEMA, Buenos Aires, Argentina) using a 20 μL reaction mixture containing 0.4 μM of each primer (Operon Eurofins, Louisville, KY, USA), 200 μM of each deoxyribonucleotide triphosphate (INBIO Highway, Buenos Aires, Argentina), 0.02 U/μL of Q5 High-Fidelity DNA Polymerase (NEB, Whitby, ON, Canada), 1 PCR buffer (NEB, Whitby, ON, Canada), 1X PCR Enhancer (NEB, Whitby, ON, Canada) and ~100 ng of genomic DNA. .. Positive (DNA from A. marginale Mercedes reference strain) and negative (MiliQ ® water, Direct-Q 5 UV, Millipore, Merk, Burlington, MA, USA) controls were included in the assay.

Article Title: A CACTA-like transposon in the Anthocyanidin synthase 1 (Ans-1) gene is responsible for apricot fruit colour in the raspberry (Rubus idaeus) cultivar 'Varnes'.
Article Snippet: .. All PCR reactions were performed in a final volume of 25 μL containing 2 ng genomic DNA, 1 × PCR buffer (NEB), 1.5 mM MgCl2, 0.2 mM of each dNTP, 0.2 μM of each primer, and 0.5 U of Phusion high-fidelity DNA Polymerase (NEB). ..

Article Title: Rhipicephalus microplus and Its Impact on Anaplasma marginale Multistrain Infections in Contrasting Epidemiological Contexts
Article Snippet: .. Molecular amplifications were performed on a T21 thermal cycler (IVEMA, Buenos Aires, Argentina) in a 50 μL reaction mixture containing 0.4 μM of each primer (Operon Eurofins, Louisville, KY, USA), 200 μM of each deoxyribonucleotide triphosphate (INBIO Highway, Buenos Aires, Argentina), 0.02 U/μL of Q5 HighFidelity DNA Polymerase (NEB, Whitby, ON, Canada), PCR buffer (NEB, Whitby, ON, Canada), PCR Enhancer (NEB, Whitby, ON, Canada) and ~200 ng of genomic DNA. .. An aliquot of each amplified product (15 µL) was analyzed by electrophoresis in 2% agarose gel (INBIO Highway, Buenos Aires, Argentina) stained with ethidium bromide (INBIO Highway, Buenos Aires, Argentina) and a molecular size marker (1 kb DNA ladder, NEB, Whitby, ON, Canada).

Article Title: Rhipicephalus microplus and Its Impact on Anaplasma marginale Multistrain Infections in Contrasting Epidemiological Contexts
Article Snippet: .. Molecular amplifications were performed on a T21 thermal cycler (IVEMA, Buenos Aires, Argentina) using a 20 μL reaction mixture containing 0.4 μM of each primer (Operon Eurofins, Louisville, KY, USA), 200 μM of each deoxyribonucleotide triphosphate (INBIO Highway, Buenos Aires, Argentina), 0.02 U/μL of Q5 High-Fidelity DNA Polymerase (NEB, Whitby, ON, Canada), 1 PCR buffer (NEB, Whitby, ON, Canada), 1X PCR Enhancer (NEB, Whitby, ON, Canada) and ~100 ng of genomic DNA. .. Positive (DNA from A. marginale Mercedes reference strain) and negative (MiliQ® water, Direct-Q 5 UV, Millipore, Merk, Burlington, MA, USA) controls were included in the assay.

Article Title: A CACTA-like transposon in the Anthocyanidin synthase 1 ( Ans-1 ) gene is responsible for apricot fruit colour in the raspberry ( Rubus idaeus ) cultivar ‘Varnes’
Article Snippet: .. All PCR reactions were performed in a final volume of 25 μL containing 2 ng genomic DNA, 1 × PCR buffer (NEB), 1.5 mM MgCl 2 , 0.2 mM of each dNTP, 0.2 μM of each primer, and 0.5 U of Phusion high-fidelity DNA Polymerase (NEB). ..

Article Title: Integrative taxonomy of the genus Longidorus (Nematoda: Longidoridae) reveals two new species in the Mediterranean Basin
Article Snippet: .. Briefly, individual nematodes were cut with a scalpel in a 20 μL drop of PCR buffer (ThermoPol ® , Biolabs, New England, USA), to which 2 μL of proteinase K (600 μg/mL) was added. ..

Article Title: Rhipicephalus microplus and Its Impact on Anaplasma marginale Multistrain Infections in Contrasting Epidemiological Contexts
Article Snippet: .. Molecular amplifications were performed on a T21 thermal cycler (IVEMA, Buenos Aires, Argentina) in a 50 μL reaction mixture containing 0.4 μM of each primer (Operon Eurofins, Louisville, KY, USA), 200 μM of each deoxyribonucleotide triphosphate (INBIO Highway, Buenos Aires, Argentina), 0.02 U/μL of Q5 High-Fidelity DNA Polymerase (NEB, Whitby, ON, Canada), PCR buffer (NEB, Whitby, ON, Canada), PCR Enhancer (NEB, Whitby, ON, Canada) and ~200 ng of genomic DNA. .. An aliquot of each amplified product (15 μL) was analyzed by electrophoresis in 2% agarose gel (INBIO Highway, Buenos Aires, Argentina) stained with ethidium bromide (INBIO Highway, Buenos Aires, Argentina) and a molecular size marker (1 kb DNA ladder, NEB, Whitby, ON, Canada).

Article Title: Systematic revision of Paralongidorus (Nematoda: Longidoridae) based on molecular and morphological evidence, with the description of a new species from Spain
Article Snippet: .. Briefly, individual specimens were cut with a scalpel in a 20 μL drop of PCR buffer (ThermoPol®, New England Biolabs, USA), to which 2 μL of proteinase K (600 μg/mL) was added. ..

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